Whole-genome sequencing analysis of imported SARS-CoV-2 cases at Hangzhou Port
DOI:
https://doi.org/10.63313/AERpc.9104Keywords:
Hangzhou Port, imported SARS-CoV-2, whole-genome sequencing, viral mutation, epidemic prevention and controlAbstract
This study employed the Nanopore nanopore sequencing platform and the Baiyi whole-genome analysis software to perform whole-genome sequencing on 50 imported COVID-19 cases collected at the Hangzhou port from September 2022 to January 2023, analyzing the distribution of viral variants to provide theoretical support and practical guidance for epidemic prevention and control. A total of 50 complete SARS-CoV-2 genome sequences were obtained, with 46 cases exhibiting sequence coverage exceeding 99%. All Pangolin phylogenetic analyses identified the variants as Omicron strains, consistent with the globally dominant variant. The distribution of these variants in the functional gene regions may influence the virus's infectivity, pathogenicity, and other biological characteristics. This study holds significant implications for COVID-19 prevention and control at the Hangzhou port and nationwide, offering theoretical support and practical guidance for optimizing detection protocols and enhancing flight controls in high-risk areas.
References
[1] Lin Q; Huang Zhimiao; Zeng Zhiwei; Chen Wei; Chen Mingjian; Weng Yuwei. Identification of SARS-CoV-2 using combined second-and third-generation sequencing technologies [J]. China Journal of Zoonotic Diseases, 2023,39(11):1053-1059.
[2] Li Jianxiong; Shi Yong; Xu Gang; Xiao Dajin; Liu Shiwen; Xiong Ying; Gong Tian. Research on rapid identification of COVID-19 cases based on nanopore-targeted whole-genome sequencing technology [J]. Experimental and Diagnostic Medicine, 2024,42(2):131-133.
[3] Yang Xinxin; Chen Zhiwei; Chen Minhong; Jia Haimei; Zhang Xiaoyang. Transmission chain and genetic sequence analysis of a COVID-19 Omicron BA.2.3.7 infection caused by cold-chain products [J]. China Journal of Zoonotic Diseases, 2024,40(4):384-390.
[4] Zhou Jiansong; Wu Ruoyu; Jian Jie; Jiang Jiajun; Li Sha; Li Wanyi; Zhou Linlin. Whole-genome sequencing analysis of asymptomatic cases infected with the Omicron variant of SARS-CoV-2 [J]. Sichuan Journal of Physiological Sciences, 2023,45(7):1129-1132.
[5] Wang Yin; Huang Yao; Zhang Jun; Li Xinnan; Zhou Le; Zhu Weiwei; Yang Qinggui; Xu Qin; Kong Guimei. Epidemiological and Genetic Characteristic Analysis of Two Cases of Novel Coronavirus Pneumonia [J]. Journal of Practical Clinical Medicine, 2022,26(19):76-82.
[6] Cai Linlin; Liang Jianpeng; Chen Libin; Xiang Bin; Ren Tao. Research Progress on Detection Methods for the Novel Coronavirus [J]. Guangdong Animal Husbandry and Veterinary Science and Technology, 2022,47(2):43-50.
[7] Kou Meiling; Xie Jiarui; Yang Jiaping; Miao Haisheng. Whole-genome sequencing and genetic evolutionary analysis of bovine coronavirus [J]. Advances in Veterinary Medicine, 2022,43(10):1-7.
[8] Zeng Yong; Chen Jianwei; Cui Enhai; Hu Guohai; Wang Xiaoguang; Ji Qiaoying; Cong Ying; Yang Yang; Yang Weibin. Genomic sequencing and variation analysis of SARS-CoV-2 in 12 overseas Chinese infected cases [J]. Laser Biology Reports, 2021,30(4):305-315.
[9] Cao Wenjie; Liu Jie; Zhang Bengong. Application Analysis of Big Data and Artificial Intelligence Technologies in COVID-19 Epidemic Prevention and Control [J]. Journal of Wuhan Textile University, 2021,34(1):10-14.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 by author(s) and Erytis Publishing Limited.
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
